Examples of chloroform

Example from the Hansard archive. Contains Parliamentary information licensed under the Open Parliament Licence v3.0
The samples were mixed well with 75 l chloroform and centrifuged for 20 min at 15 000 rpm.
Mineralization of bacteria and fungi in chloroform fumigated soil.
The aqueous phase was transferred into an autoclaved 1.5 ml microcentrifuge tube and the chloroform : isoamyl step was repeated.
Two phenol : chloroform and one chloroform :isoamyl alcohol (24 : 1) extractions were then performed.
A year later he died, without benefit of chloroform.
The neutral lipids were eluted with 20 ml chloroform, followed by elution of the polar lipids with 30 ml methanol.
Extracts were evaporated to dryness in vacuo, reconstituted in water, chloroform added (1:1 v/v), vortexed, centrifuged, and the aqueous layer recovered.
The supernatants were extracted by phenol or phenol/chloroform and precipitated by ethanol.
Samples were extracted with one-third volume of chloroform: isoamyl alcohol (24:1) mix and centrifuged to separate phases.
The chloroform-bottle is constructed so that the liquid comes out in single drops.
At the time, ether and chloroform were being tried as anaesthetics, so the proponents of mesmerism may have faced some jealous rivals.
Glycolipids were dissolved at approximately 2 g\\l in chloroform\\methanol\\ water (10 : 10 : 1 v\\v\\v).
Here were a wooden operating table and chloroform and ether apparatus.
Three samples of five embryos (c. 1 g) each were extracted with chloroform : methanol [1 : 2 (v/v)].
A phenol 0 chloroform extraction was used to purify the linearized plasmid.
The reaction was stopped and the lipids extracted with chloroform.
The chloroform layer was collected and used for the determination of phospholipids and sterols.
The sample was extracted once with an equal volume of chloroform (24 : 1 [v/v] chloroform : isoamyl alcohol).
Assessing biological soil quality with chloroform fumigation-incubation: why subtract a control?
The observation of clear oscillations demonstrated the formation of specific aggregates with fixed distances between the spin labels within a chloroform/toluene mixture.
Extracts were poured into brine twice and extracted with chloroform.
The mice were exsanguinated under chloroform anaesthesia.
705 noys fixative (10% acetic acid, 30% chloroform, 60% ethanol) for 2 h, after which they were dehydrated over several hours and embedded in paraffin in transverse orientation.
Samples of the raw sewage were concentrated about 100-fold, and chloroform extracts of the concentrates were assayed for infectious virus using poliovirus susceptible cell lines.
After centrifugation the pellet was washed successively with 2 ml of distilled water and 2 ml of methanol and then suspended in 2 ml chloroform/methanol (1:1, v/v).
If it is impracticable to breed the fleas in this manner, the nest may be shaken up in a linen or paper bag containing several drops of chloroform or ammonia.
The supernatant was washed first with one volume of phenol - chloroform - isoamylalcohol [25:24:1 (v/v/v)] solution and then with one volume of chloroform, and designated as sup1.
Three subsequent extractions were carried out using a monophasic system, methanol: chloroform: water, 2:1:0-8 containing 0-005 % of the anti-oxidant, 4-methyl-2, 6-di-tert-butylphenol.
At the end of their 66th year, men and women are admitted to a college for a 12-month period of preparation for euthanasia by chloroform.
The rats were anaesthetized using chloroform.
After centrifugation, the aqueous phase was extracted three times with chloroform to remove phenol, then precipitated with two volumes of ethanol in the presence of ammonium acetate.
The usual method is to administer an overdose of chloroform.
From the
Hansard archive