| 词汇 | example_english_culture-medium |
| 释义 | Examples of culture mediumThese examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. An improved culturemedium supports development of random-bred 1-cell mouse embryos in vitro. Culturemedium was changed every day and the growth and development of embryos and colonies were examined daily. Immediately after the detection of a single trypanosome in the drop, 0-1 ml of fresh culturemedium was rapidly added to the well. The wells were overlaid with a mixture of agarose and tissue culturemedium and incubated for 7-13 days. Cleaved embryos were transferred to fresh culturemedium supplemented with glucose (5 mg/ml) and incubated for another 2 days to the morula/blastocyst stage. Fertilized eggs are transferred into a special culturemedium for further growth. Immediately after a daily motility score was recorded, each worm was transferred daily to a new well containing fresh culturemedium containing extracts. The rate of uptake of glucose and pyruvate can be related to their respective concentrations in the culturemedium. A few specimens were lost during transfer between different culture media or between culturemedium and microscopic slides. These differences in phosphorylation might be due to different maturation conditions, especially the different culturemedium additives. Pellets were prepared with the fungus mycelia in liquid culturemedium and contained approximately 20% fungus. Tachyzoites (4i10%) were incubated for 30 min in cell culturemedium with decreasing concentrations of serum. Nuclear-cytoplasmic 'tug of war' during cloning: effects of somatic cell nuclei on culturemedium preferences of preimplantation cloned mouse embryos. Before culture, the oocytes were washed three times in a maturation culturemedium. Prior to inoculation, larvae were suspended in physiological saline after repeated washings with culturemedium. The medium was replaced with the above described cell culturemedium with 50 mg/ml gentamicin. Other changes in the culturemedium resulted in reduced moulting and survival times compared with the pepsin-added media. In both experiments, half the volume of the culturemedium was exchanged for fresh medium every 4 days. Aliquots of 0.5 ml culturemedium were placed only within the inserts. To each well, 1.5 ml of culturemedium was added followed by 10 ml of the appropriate compound solution. Perfusion is represented by an imposed flow of culturemedium. Before use in proliferation or stimulation assays, parasites were harvested by centrifugation and resuspended in fresh culturemedium. The culturemedium was replaced every 2 days until the cells proliferated to confluency. The culturemedium is also an important factor in the colony formation of blastocyst-derived cells. The second control was performed to assess the influence of a putative inhibitory substance secreted by granulosa cells into culturemedium. Capacitation of hamster spermatozoa with the divalent cation chelators d(-)penicillamine, l-histidine and l-cysteine in protein free culturemedium. Medium was replaced every 2 days with freshly prepared culturemedium. Medium was changed every other day for freshly prepared culturemedium. A small proportion of dying infected cells (20%) was observed only when the parasites were beginning to be released into the culturemedium (96 h). The culturemedium was not changed during the incubation. Second-stage larvae were then collected aseptically and maintained in a culturemedium until use. The nidus was culturable in cell culturemedium and appeared to contain lipopolysaccharide. The culturemedium was changed every 3 days. This population was diluted to 3 x 103 trypanosomes/ml in culturemedium. Neuronal injury in the cell cultures resulting from glutamate excitotoxicity is assessed by quantifying the release of lactate dehydrogenase into the culturemedium. We also prepared comparable series of embryos using both culturemedium and growth within the egg in which no marking was performed. While some culturemedium components augment embryo development in vitro, others have been found to induce development arrest. It was therefore logical to address the possibility that differences in culturemedium could account for these conflicting results. These modifications were achieved by washing spermatozoa by high-speed centrifugation and the replacement of the supernatant with fresh culturemedium used for capacitation. The authors found that doses of caffeine higher than 450 g/ml in the culturemedium inhibited oocyte maturation. In control experiments, oocytes were injected with culturemedium. Immediately after the microinjection, the oocytes were cultured for 15-20 min in culturemedium to allow binding of the drug to the receptors. Platelet-derived growth factor is detected in human blastocyst culturemedium but not in human follicular fluid - a preliminary report. Effect of glucose in the culturemedium on development of horse oocytes matured and microfertilized in vitro. Every 2 days, the culturemedium was replaced by fresh medium. Trypsin-like hatching protease from mouse embryos: evidence for the presence in culturemedium and its enzymatic properties. Various doses were added directly to the culturemedium in a constant volume of vehicle (5 l). The culturemedium was replenished every other day with freshly prepared medium. The rest of the components were dissolved directly in culturemedium. Progesterone was dissolved in ethanol and added (5ul) directly to the culturemedium to give a final concentration of 1 jag/ml. On the day of use, an aliquot was thawed and diluted in the culturemedium to the desired working concentrations. Culturemedium conditioned by porcine granulosa also enhanced the maturation rate, and this beneficial effect was not diminished upon freeze-thawing. The basal culturemedium was variously supplemented to provide the required concentrations of these sulphur-containing amino acids. Nuclear-cytoplasmic "tug of war" during cloning: effects of somatic cell nuclei on culturemedium preferences of preimplantation cloned mouse embryos. The biofilms were thus exposed to a positive pressure from the culturemedium. This was accompanied by visible foaming of the culturemedium. The bioreactor system is modelled as a two-dimensional channel containing a tissue construct through which a flow of culturemedium is driven. Specifically, we consider the response of the cells to their local density and the culturemedium pressure. Gangliosides were then diluted using the culturemedium and filtered as described above. The organisms were then diluted to 1 x 102 or 6-3 x 103/ml in fresh culturemedium. The final volume of culturemedium was adjusted to 0-2 ml/well by adding fresh medium. The culturemedium (1 ml) was placed into each well with 1 parasite. It might be possible that the toxin concentration in the culturemedium was too low to show its activity. Evidently, the changing nutritional requirements of developing worms were not being catered for by the utilized culturemedium. The liquid culturemedium was used to dilute the basal gel to create 60%, 50% and 40% diphasic gel/medium. Worms were washed repeatedly to remove culturemedium prior to extraction. Culturemedium was not changed during this period. Reagents were added (5 l) directly to the culturemedium. This difference might be due to the different composition of the culturemedium. After collection, the follicles were transferred from the collecting medium into culturemedium. Such developmental blocks can give rise to resistance to the components of the culturemedium and culture condition. These oocytes were placed in culturemedium until required. Following several washings with culturemedium, oocytes were used for fluorescence labelling studies. In the present study, we found that the culturemedium did affect polymerisation of actin filaments and may regulate embryo development under in vitro conditions. On days 2 and 4 of culture, 25 l of fresh equilibrated culturemedium was added to the culture drops. The addition of several factors to the embryo culturemedium can modulate in vitro embryo development. The concentration of these growth factors clearly is influenced by the presence of several embryos in the culturemedium. The addition of growth factors to the culturemedium may have practical implications. The same culturemedium was used for preparing serum, which was used at a final concentration of 105/ml for insemination. An improved culturemedium supports development of random bred 1-cell mouse embryos in vitro. Overcoming the 2-cell block by modifying standard components in a mouse embryo culturemedium. Overcoming the 2-cell block by modifying standard components in a mouse culturemedium. Autogamous cells were isolated in fresh culturemedium, and phenotypes were observed after they had undergone 10 cell divisions. The culturemedium was replaced with serum-free medium 24 hours before all drug treatments. Further dilutions of each of the drugs were made in culturemedium. The culturemedium was then collected from each well, and 2 ml fresh medium was added, using infrared illumination. This would include lowering the temperature, obscuring the bulk of the embryo or using a culturemedium with reduced energy conductance. These examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. |
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