| 词汇 | example_english_aliquot |
| 释义 | Examples of aliquotThese examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. Sugars were extracted with four successive 250 l aliquots of 80% (v/v) ice-cold ethanol and further grinding. Each pool was divided in two equal aliquots and processed in duplicate. Due to the small amount of material available, only 15 aliquots of the sample could be prepared by settling in acetone on aluminum discs. Two 100 ml aliquots were taken from the vials and the number of nematodes contained in 100 ml was determined under stereo-microscope. If helminth eggs were found in any of the five aliquots of a given sample, this was recorded as 'contaminated'. Further serial dilutions of the cercarial suspension were made to give five aliquots having densities of 20, 15, 10, 5 and 2.5 cercariae ml21. A suspension of eggs was fertilised at time 0 and aliquots fixed at the indicated time points. The drug solutions were prepared from frozen aliquots of stock solution, dissolved in extracellular solution, and applied from seven-barrel pneumatic puffer pipettes. The supernatants were divided in two aliquots for further analysis. At the start and after 5 h of incubation, aliquots were removed and analysed. The remaining homogenate was centrifuged for 2 min at 10,000 g before aliquots of supernatant were removed for the following assays. Positive results were confirmed by repeating the assay using two aliquots from the same sample. The bags were checked daily for evidence of green colour and aliquots were removed for absorbance spectra after 8 days. Two percent aliquots of the total worm burden were counted. The total number of eggs was calculated by counting eggs in multiple representative aliquots of each collection. Samples were then split into several aliquots and loaded into individual 1.5 mm diameter holes in a copper planchet. The remainder of the suspension was diluted 2-fold and 100 ml aliquots were deposited into three 96-well plates. Equal aliquots from the homologous sera were pooled. At different times, 1 ml aliquots were rapidly centrifuged and the eggs pellets were frozen. In 1993 duplicate aliquots from the same faecal sample were counted for the fourth, fifth and sixth sampling time. Larval initial dry weight was estimated by the same means used to estimate leaf initial dry weight (scatterplot regression of 20 aliquots larvae each year). From each sample, three aliquots of 50 ml were examined microscopically for the identification and counting of eggs/larvae. Then 0n2 ml aliquots of the incubation medium (containing 123-133 schistosomula) were taken at 2 h intervals. Sequential dilutions of this insert were prepared and stored in aliquots for later use. Parasites were enumerated by calculating the mean number in 3r5 ml aliquots of the washed sporocyst preparation. Fractions of 1 ml were collected in scintillation vials and aliquots of these solutions were assayed for radioactivity as described above. Ten microliter aliquots were spotted at 1 min intervals onto nitrocellose filter membranes attached to a vacuum manifold. Firstly, supernatants of phenol extracts were divided into two aliquots. The supernatants were divided into two aliquots: one half was directly counted for total radiolabelling, the other for conversion into amino acids. The wash water was divided into 50 ml aliquots and treated by the same methods as described above, except for the method based on positive larval thermo-hydrotropism. Ten 5 ml aliquots were taken from the corresponding wells and deposited on a slide for microscopical observation at 24, 48, 72 and 96 h post-exposure. Specimens of the modern fern and aliquots of the biochemicals have been anoxically thermally degraded to simulate the alteration that occurs during diagenesis and resultant fossilization. In one series of experiments, aliquots from each of the two lots were transferred to a perfusion chamber, and membrane potential measurements were carried out on individual impaled eggs. When there were sufficient worms for identification purposes (100 individuals), one or two more aliquots (up to a total of 30% of the volume) were analysed. At the end of the collection period, the pooled aliquots of the wet faeces for each animal were weighed, mixed well and analysed for nitrogen concentration. At 35 days post-infection, faeces from lambs infected with the same isolate were thoroughly mixed and aliquots of 10 g containing approximately 3000 epg were dispensed in plastic beakers. Worms were differentiated with respect to species and developmental stage, and counted in 20 ml aliquots of the wash/digest with a minimum sensitivity of 1-100. The contents and washings were made up to 2 l, from which ten 4 ml aliquots were examined to estimate the size of the adult nematode population. We have found such small changes could only be reliably detected using automated titrators injecting and/or removing small aliquots of sample into a stirred cuvette. The spores contained in the homogenate were quantified (20 l aliquots) in a haemocytometer, and the results then expressed as number of spores per mg of muscle. Each column was then leached repeatedly with 75-ml aliquots of distilled water in an attempt to bring both soils to a common low concentration of nutrients in solution. The pool of probes was aliquoted into four tubes for four different reactions. From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. The 5 ml aliquot of the 10 times diluted scytonemin fraction was injected and data were acquired for both positive and negative ionization modes. Consequently, the present experiment was designed to minimize and standardize this effect and each aged aliquot was used only once. A 1 ml aliquot of cercarial suspension (3 h post-emergence) of known density was added to each ring. Viability tests involved streaking of an aliquot of the suspension on sterile plates containing the appropriate nutrient medium for each species. Furthermore, the anisotropy of the donor can be determined in the presence and absence of acceptor dye molecules in an aliquot of identical cell population. After the first cycle a second aliquot is added, after the second cycle a third, etc. so building up a ladder. The spectral properties of an air-dried aliquot of this sample contain features of both minerals and favour those of ferrihydrite for the finer fraction. Oxidation of linoleic acid was measured as the absorbance of an aliquot of the test emulsion at 232 nm. A 5-ml aliquot of the original medium was removed and counted. An aliquot was removed to determine the uptake of the radioactive precursor. To a 250 l aliquot, 10 g/ml heparin was added. The control stimulus was a similar pipette containing a filter paper impregnated with a 10-ml aliquot of hexane. An aliquot of the sediment was examined for strongyle eggs. Both motile and immotile worms in 10 ml aliquot of each sample were counted daily without changing the medium for 7 days. Each aliquot contained enough seeds (at least 125) to permit water content and germination assessment. A 250 l aliquot of this suspension was added to each fertilisation well to obtain a final concentration of 1 x 106 spermatozoa/ml. On the day of use, an aliquot was thawed and diluted in the culture medium to the desired working concentrations. An aliquot of the product was run out on an agarose gel to determine efficiency of amplification. Potassium was determined on a separate aliquot of the sample by flame photometry. The soil-methanol mixture was filtered and an aliquot (5-25 l) of the extract used for subsequent analysis as described above. Usually an aliquot sample is suspended in an iso-osmotic saline solution and spread on nutrient agar plates. An aliquot was removed for counting and microscopic examination for bacteria. Mixtures of retinal1 and retinal2 standards were co-chromatographed with an aliquot of retinal oximes to identify isomers. Following placement of the catheter, a 50 ml aliquot of sterile isotonic saline (0.9%), warmed to body temperature, was slowly infused. One aliquot, representing 10% of the volume of the ingesta was examined in small portions under a stereoscopic microscope to collect the nematodes. A 100 ml aliquot of each bacterial cell suspension (mean standard inoculum of 6.7r107 c. f. u.) was added to respective organ cultures. Following incubation, the tube was allowed to cool or warm to room temperature, and an aliquot of the eggs removed for 'mechanical hatching'. An aliquot (25 l) of each inhibitor (10 mg\\ml) was serially diluted with an equal volume of saline in microtitre trays. An aliquot (20 l) of each incubation mix was then subjected to gelatin substrate gel electrophoresis as described above. An aliquot (20-40 g/ml) of the sialoglycoprotein described above was added to the supernatant in a test tube, and then mixed by vortexing. In many cases, the falsepositive rate can be dealt with effectively by simply re-testing an independent aliquot of the sample to confirm a positive result. Then they would take aliquots of the sample to test for the antimicrobial activity of a specific drug or protein (antimicrobial peptides). From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. After the final dilutions are produced, aliquots of the suspension are plated or placed in wells and incubated. From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. Every 24 h the water in the pot was swirled and a 1 ml aliquot of the water was taken and put in a watch glass using a pipette. An aliquot of the probe (5 ml, 100 mg) was mixed with 5 ml of sterilized, deionized distilled water, boiled for 5 min and chilled immediately in ice. Aliquots of 100 l of these different drug dilutions are added to the appropriate microtiter wells already containing 100 l of medium, resulting in the required final drug concentrations. From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. Each aliquot is divided into two portions. When a blood vessel supplying tumor has been selected, alternating aliquots of the chemotherapy dose and of embolic particles, or particles containing the chemotherapy agent, are injected through the catheter. From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. The host cell suspension was divided into small aliquots (approximately 2-5 ml each), one aliquot for every 3 to 4 g of original wet weight. An aliquot of the extracted proteins was transferred to clean 1.5 ml microcentrifuge tubes, centrifuged at 11000 g for 20 min to remove residual sporocysts bodies and insoluble debris. Both flame photometry and mass spectrometry are destructive tests, so particular care is needed to ensure that the aliquots used are truly representative of the sample. From Wikipedia This example is from Wikipedia and may be reused under a CC BY-SA license. One aliquot was processed with zinc sulphate at density 1180, another with zinc sulphate at density 1420, and the third aliquot using a saturated sugar solution of density 1240. Five μl aliquots were resolved on a 1.3% agarose gel. Counts were accumulated by scintillation spectroscopy for duplicate aliquots from each of the triplicate experimental samples. Four to five aliquots of each type of sample were used for the exposure experiments. To estimate the total larval number, five 20-ml aliquots of larval solution were counted and total larvae in the suspension were estimated. A logistic regression is insensitive to differences in the number of cercariae per treatment; 20 -62 cercariae were present in the 0.5 ml aliquots. Adding multiple aliquots of enzyme during the incubation period also had no effect. Two aliquots from each mouse were analysed in a haemacytometer. Division of the whole suspension into aliquots, each of which was used once only, was carried out to minimize mechanical damage to cercariae. Worm numbers were estimated from pooled aliquots of contents and digests and 50 worms measured from each individual. Nutrient loading of each soil sample consisted of adding sequentially three aliquots of 75 ml of nutrient solution. The lysed specimen was then centrifuged at 7000g for 5 min and the supernatant divided in two aliquots. The stock solution was frozen in aliquots until used. All the positive fractions were pooled together and aliquots from the pool were processed for densitometry. These examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. |
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