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The samples were mixed well with 75 l chloroform and centrifuged for 20 min at 15 000 rpm.
Mineralization of bacteria and fungi in chloroform fumigated soil.
The aqueous phase was transferred into an autoclaved 1.5 ml microcentrifuge tube and the chloroform : isoamyl step was repeated.
Two phenol : chloroform and one chloroform :isoamyl alcohol (24 : 1) extractions were then performed.
A year later he died, without benefit of chloroform.
The neutral lipids were eluted with 20 ml chloroform, followed by elution of the polar lipids with 30 ml methanol.
Extracts were evaporated to dryness in vacuo, reconstituted in water, chloroform added (1:1 v/v), vortexed, centrifuged, and the aqueous layer recovered.
The supernatants were extracted by phenol or phenol/chloroform and precipitated by ethanol.
Samples were extracted with one-third volume of chloroform: isoamyl alcohol (24:1) mix and centrifuged to separate phases.
The chloroform-bottle is constructed so that the liquid comes out in single drops.
At the time, ether and chloroform were being tried as anaesthetics, so the proponents of mesmerism may have faced some jealous rivals.
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