词汇 | agarose |
释义 | BETA Examples of agaroseagarose isn’t in the Cambridge Dictionary yet. You can help! Resolved on a 2 % agarose gel and visualized with 10 g\\ml ethidium bromide. The solidified agarose was cut into blocks (about 1 mm3). The samples were run on agarose gels and blotted to nitrocellulose. Once the vitelline envelope was removed, the eggs became very fragile and easily damaged; an agarose pad for support and minimal handling were required. For the purification step, a large number of systems that used agarose were commercially available. Fragments were separated through electrophoresis on 2 % agarose gels and visualized using ethidium bromide under ultraviolet light. The products of digestion were analysed on 2 % (w/v) agarose gels. The band definition was adequate for confident visual comparison of the fingerprints located on the same or on different agarose gels. This phenomenon may explain the presence of bands in agarose gels from all 3 species which appeared longer than the sequences obtained. After amplification, 10 ml of the amplification products were electrophoresed on a 2% agarose gel and stained using ethidium bromide. Amplification products were separated on a 2 % agarose gel and stained with ethidium bromide. Amplification of each gene-specific fragment was confirmed by examination of melting peaks and by agarose gel electrophoresis. Conditions were optimized by varying amplification conditions until only a single clear band was visible when the products were electrophoresed on a 2 % agarose gel. Separation of fragments was carried out by agarose gel electrophoresis (0n8 %). Restriction fragments were visualized on 3% agarose gels containing 1% ethidium bromide. These fragments were then identified on agarose gels as described below. The wells were overlaid with a mixture of agarose and tissue culture medium and incubated for 7-13 days. The products of the digestion were separated by gel electrophoresis in 2.8 % agarose and stained with ethidium bromide. |
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