Examples of transfection

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The transfection rate in the cells, as assessed by expression of b-galactosidase, was approximately 50%.
However, these alterations were not detectable in the primary tumour, and rather must be categorised as transfection artefacts.
The intrabodies can be synthesized by the cells expressing multi-subunit receptor structures after gene transfection.
The major problem associated with plasmidbased gene delivery to skeletal muscle is the relatively low efficiency of transfection.
Neuronal transfection in brain slices using par ticle-mediated gene transfer.
This indicates the negative effect of transfection on embryo development in vitro.
In summary, the results obtained so far show that transfection of the mouse embryo can be used to obtain transgenic mice.
For effective somatic gene therapy, the major obstacle is in achieving efficient transfection of the correct cells to provide an appropriate level of gene expression.
This implies suboptimal transfection would be sufficient, provided stable expression was achieved, to produce a cure.
Precisely which of these represents the most transfection-efficient fraction is not clear.
This allows for transfection of the both neural tube and premigratory neural crest cells.
Several methods have thus been developed to transfer genetic material into target cells, a process termed gene transfection.
Nonviral vector systems have low transfection efficiencies, and most of the viral systems have the problems of poor tumour targeting, immunogenicity and low transduction efficiencies.
This efficiency was not diminished by transfection with marker genes, selection and passage of the cells.
Cells were harvested 48 h after transfection.
However, we need to interpret the results from cell transfection assays with caution because recombinant protein expression is usually at higher levels than endogenous levels seen in vivo.
Taken together our results show that lipid transfection can generate transgenic mice, but the efficiency needs to be improved for this method to be widely applied.

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