Examples of supernatant

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The supernatants were collected and dried under vacuum.
After 4 days, the supernatants were collected for cytokine determination.
After centrifugation, the supernatant solution was assayed for the above components.
Percentage release of isotope into the supernatant fluid was then calculated.
Each plate contained the original faecal supernatant before centrifugation, after centrifugation, and after filtration as a positive control.
Then 1 ml of supernatant was added directly to each preenrichment medium (9 ml).
After the extraction, the suspension was centrifuged at 12 000 g for 15 min and the supernatant analyzed for carbohydrate and protein.
Immediately prior to measurements, the supernatant was evaporated to dryness under nitrogen and the tocopherol was redissolved in methanol.
The supernatant was collected and the pellet was re-extracted twice more with 80% ethanol.
Immediately prior to measurement, the supernatant was evaporated under nitrogen and the tocopherol was redissolved in methanol.
To remove cells and debris, the follicular aspirate was centrifuged at 1000 g for 10 min and the supernatant passed through a 0.5 m filter.
The lysed specimen was then centrifuged at 7000g for 5 min and the supernatant divided in two aliquots.
After 40 min of incubation at room temperature, the supernatant surface film was removed and postfixed as described above.
The supernatant was filtered in the presence of polyvinyl polypyrrolidone and activated carbon to eliminate pigments and polyphenols.
The homogenate was centrifuged and the supernatant concentrated to 500 mg/ml as before.
The supernatant was extracted with 500 ml of chloroform : isoamyl alcohol (24 : 1) by centrifugation at 14,000 rpm for 20 min to separate the phases.
Bacterial cells were harvested by centrifugation (20 800 g, 3 min), and the supernatant was then removed.
The supernatant was used as antigen and uninfected cells, similarly treated, were used as control antigen.
The culture was then centrifuged at 25 000x g for 15 min and the supernatant was discarded.
The assay suspensions were centrifuged at 15000rpm for 5 min and the supernatants discarded.

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