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This indicated that maize callus produced raffinose in response to some, perhaps all, stresses, but that raffinose amounts may be controlled by hydrolytic activity.
However, these reserves are unavailable to the embryo until processed through the action of hydrolytic enzymes.
Hydrolytic enzyme activity is stronger in highly virulent strains.
They hydrolyse the 1,3;1,4- -glucans and provide access for other hydrolytic enzymes during the postgermination mobilization of storage reserves.
They also act to stabilize cytoplasmatic organelles, thus avoiding liberation of hydrolytic enzymes, limiting in this way the inflammatory process.
Both the scolex tegument and glands are positive to tests for hydrolytic enzymes like acid and alkaline phosphatases.
In different seeds, the syntheses of the three hydrolytic enzymes occur in different places, and mobilization of galactomannan occurs in different ways.
The source of hydrolytic enzymes that digest starchy endosperm reserves is largely from both the scutellum and the aleurone.
Furthermore, these enzymes are unique compared to other hydrolytic endonucleases in that the basic residues in their active sites are not generally found in contact distance with metal-bound waters.
E-64, a cysteine proteinase inhibitor, completely inhibited these hydrolytic activities (data not shown).
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