| 词汇 | example_english_stain |
| 释义 | Examples of stainThese examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. The product was analyzed by agarose (2%) gel electrophoresis and stained with ethidium bromide. We have previously demonstrated that staining for intracellular cytokines correlated with cytokine secretion. The protocol is especially robust with respect to absence of background staining that can limit assay interpretation. Specimens were cut longitudinally to the midline, and 5-m sections mounted for staining with hematoxylin and eosin or toluidine blue. In each assay, five categories of staining were observed as defined previously. The staining intensity was categorically coded as 0 (absent), 1 (weak), 2 (moderate), or 3 (strong). In the progression from normal mucosa to metaplasia to dysplasia, the results obtained with both antibodies suggested differences in staining frequency and intensity. We found minor differences in the staining pattern between methanol and paraformaldehyde fixed cells, but immuno-labeled nuclear structures were visible regardless of fixation. The sections were stained with antibodies for those two antigens. Nerves estimated to contain greater than 25 dark staining axons per section but still maintaining a significant portion of live axons were rated as moderate. Serial sagittal sections passing through the optic nerve were collected, stained with hematoxylin and eosin, and analyzed for pathologic changes. Sections (6 m) were cut from both faces of bisected tissues and stained with hematoxylin and eosin. There is no difference in staining pattern in regard to the subtypes of the rhabdomyosarcomas, namely embryonal or alveolar subtypes. Distribution and morphology of human cone photoreceptors stained with anti-blue opsin. Between 30 and 60 min we frequently observed parasites having surface patches on the schistosomulum body but with little or no staining on the anterior. Cestodes were stained with acetic carmine and identified under the microscope. The results showed that the glycocalyx was intensely stained by the first two dyes, thus indicating the presence of relatively high amounts of negative charges. After amplification, 10 ml of the amplification products were electrophoresed on a 2% agarose gel and stained using ethidium bromide. Amplification products were separated on a 2 % agarose gel and stained with ethidium bromide. When necessary, the amplification products were further analysed on 6 % polyacrylamide gel stained with 0.1 % silver nitrate. Horizontal bedding strata are crazed with minute shear fractures then stained by seeping iron oxide in solution. The floor of the workshop is compacted soil and is visibly stained with oils and fuels. In such animals, all the patches appear evenly stained after monocular enucleation. Alpha 2 adrergic receptors in cilairy and retina marker which stains the plasma membrane of the soma. The darkly stained surface soil in the depot area also suggests that other petroleum products were spilled at the site. All measurements and morphological characterization were made on fixed and stained specimens. Indeed, the antibody stained the asters and (more strongly) the vesicular components attaching to the periphery of the mitotic apparatus. The staining intensity in oocytes of primordial and primary follicles was stronger than in late-staged follicles. Adjacent series of sections were stained with cresyl violet or reacted for acetylcholinesterase histochemistry for the identification of cytoarchitectonic bounderies. The uranyl acetate- and lead citrate-counterstained sections showed that the colloidal iron only stained glycocalyx but not the surface membrane. Sections (5 mm thick) were stained with haematoxylin and eosin. Specimen embedded in paraplast; 5 mm sections stained with hematoxylin/eosin. The successful introduction of any vaccination programme may be dependent on the diversity of co-circulating rotavirus stains and the dynamic nature of this diversity. Oocysts were analysed during excystation and were stained using both dyes. The gels were stained and destained as described above. The dark stained parts indicate the biotin-streptavidin complex. The fibrils were negatively stained with uranyl acetate. The chromagen reaction product was observed as diffuse staining throughout the cytoplasm, and both spindle shaped and flattened cells were seen. By the 10-somite stage, there is strong staining in the lateral ectoderm and caudal dorsal neural tube. A deeplystaining ohromatin mass lies to one side of the pink-staining body and upon or within a small spherical mass of faintly staining chromatin. The level of staining throughout the syncytium was markedly increased. Cells were fixed, and the microfilaments were stained with rhodamine-phalloidin and visualized by fluorescence microscopy. The products of the digestion were separated by gel electrophoresis in 2.8 % agarose and stained with ethidium bromide. Cells were stained to examine carbohydrate composition using several different lectins conjugated with a fluorescent chromophore. Staining for lipid alone is easier and safer than staining for protein with mercury bromophenol blue, but the latter could be used. The time from emergence to feeding can be approximately estimated from the staining experiments described on p. 578. Weathering byproducts preferentially stained echinoderm cavities providing good colour contrast on gogiid moulds compared to the matrix. Sections were stained with lead citrate and uranyl acetate. Analysis of connexon images obtained with cationic and anionic negative stains. Plates were shaken for 20 min at 60 rpm during staining. The cells are smaller and the walls remain thin and have different staining characteristics from the thick secondary walls of the lateral endosperm cells. External cracks as a result of mechanical impact were stained by dye, whereas the internal preharvest stress cracks remained unstained. The sections were stained with 2% uranyl acetate in 50% ethanol and 0.4% lead citrate solutions for 10 min each. At maturity, the entire seed coat of the wild-type seed stained red. Sectioning and staining were carried out as described for the osmium vapour fixed samples. Under these conditions, staining intensity was directly proportional to concentration and could be used as a measure of undigested substrate. Improved procedures for clearing and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. The area occupied by the nucleus was excluded, as it was less intensely stained than the cytoplasm. The fixed oocytes were stained with 1% orcein to examine in whole mount. Regular patchy distribution of cytochrome oxidase staining in primary visual cor tex of macaque monkey. Individual sections were stained for cell body (cresyl violet) and myelin. On the other hand, necrotic cells are well stained after a short incubation with propidium iodide due to extensive membrane damage. They were serially sectioned at 1.25 m and stained with toluidine blue. Again at least 80 oocytes were fixed, stained and their nuclear status was assessed. Five untreated blastocysts were used for each control staining. The lightly staining nuclei contain one or two prominent nucleoli. In a few instances, contradictory patterns of staining were found in the same animal. Moreover, positive staining reached as far as the outer limiting membrane. Adjacent sections were mounted and stained with 0.1% cresyl violet for histological identification. The purpose was to verify the viability of the antibodies and to provide a comparison of staining in a well-characterized species. Only expanded and hatching blastocysts were selected for staining. We observed the 'one dot' staining pattern exclusively in embryos up to the 2-cell stage. By 34 h already 13% of the embryos were stained according to pattern 4, corresponding to a much higher level of enzyme activity. Indirect immunofluorescence, with antibodies raised against acrosomal matrix, showed different staining patterns between acrosome-reacted and intact spermatozoa. Despite some variation in the intensity of staining between specimens, these cells could be reliably identified in the peroxidase-labeled whole mounts. The compressed oocytes were fixed in 2.5% glutaraldehyde for 2 min, stained with 1% acetocarmine for 2 min and washed in 45% acetic acid. Significant differences in the staining behaviour were not detected with the three antibodies. Slices were stained with lead citrate and uranyl acetate. To assess the rate of fertilization, the oocytes were fixed 10 h after fertilization and stained with orcein. Fresh staining solutions were prepared for each assay and protected from light by aluminium foil. Two control groups (10 oocytes per group) were used in order to assess that no staining meant absence of microtubules and microfilaments. Tissue sections (5 m) were stained with haematoxylin-eosin for histological observations. Both oocytes were stained during the same experiment. Frozen sections (40 mm) were cut tangential to the medial sur face of the lateral gyrus and stained with methylene blue-azure. Subsequently, the sections were stained with uranyl acetate and lead citrate. In fact, staining was very weak at 20 -30 mm below the sur face of the slice. In addition, these authors also demonstrated this area to be intensely stained for cytochrome oxidase and for parvalbumin, and lightly stained for calbindin. All of the above variations of the staining protocol gave consistent results. They also stained more intensely for myelin basic protein, implying that greater myelin content made the columns visible in dark field. Sagittal sections were taken every 60 mm with a freezing microtome; sections were mounted and stained with cresyl violet. However, the monoclonal antibody did not produce a comparably clear staining pattern in whole mounts. Cells in the ganglion cell layer and some inner nuclear layer were also stained. The brain was extracted, cut in serial sections (each 30 mm) in the coronal plane, and stained with cresyl violet. When stained spermatids were injected into oocytes without washing, none of the embryos developed beyond the 8-cell stage. Serial sections were then prepared and stained with haematoxylin and eosin according to standard procedures. The connecting body, as well as the proximal flagellum, are also prominently stained. The oocytes were fixed in ethanol:acetic acid and stained by aceto-orcein. To assess maximum fluorescence, embryos were stained with anti-actin (cellular). These examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. |
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