| 词汇 | example_english_incubate |
| 释义 | Examples of incubateThese examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. Retinas were incubated in primary antibody in blocking solution overnight at room temperature with gentle agitation. The gels were blotted onto nitrocellulose, the nitrocellulose cut into strips, and the strips incubated with antibodies to cytoskeletal proteins. Retinas were dissected from dark-adapted mice and incubated for 30 min in dark or ambient light. The wells were overlaid with a mixture of agarose and tissue culture medium and incubated for 7-13 days. Three embryonated chicken eggs incubated for 9 days were injected with 0.2 ml of the solution per egg. The wells were incubated for 2 h at room temperature with diluted human sera. Samples were incubated for 2 h and faecal sediments were harvested by sequential centrifugation in 15 ml test tubes. The cultures were incubated and colony diameters of the fungi were measured at regular intervals. The plate was incubated for 1.5 h at room temperature. The plates were incubated at room temperature for 60 min and washed before a substrate solution was added. Blots were incubated overnight with the various test sera. The flask was incubated for a period depending on ambient temperature. The nematode was incubated in ivermectin for a total period of 30 min ; spike activity was recorded for the last 2 min of this period. In order to investigate the mechanism of triclabendazole resistance, triclabendazole-susceptible and triclabendazole-resistant flukes were incubated in vitro with triclabendazole sulphoxide (50 g\\ml). Samples containing distilled water alone (blank) and others spiked with faecal material were also incubated in order to assess the effectiveness of the incubation phase. As a negative control, some sections were incubated without the primary antibodies and processed as described above. The tissues in panels a and c are incubated with anti-a, those in panels b and d are incubated with anti-fi. The samples were incubated under field conditions for three hours. At supraoptimal temperatures, seeds incubated in lower water potentials had a lower temperature threshold for germination. Dishes were randomized within each temperature and incubated in the dark. All seeds were incubated until no germination was observed for 10 d (after 4 weeks). Following these pre-treatments, seeds were incubated in the same conditions as mentioned previously for the cold stratification experiment. When oocytes were incubated without mineral oil for 22 h, parthenogenetic indexes were lower than those for oocytes incubated with mineral oil (p < 0.01). The three preparations incubated in normal saline were added to the first group. Cleaved embryos were transferred to fresh culture medium supplemented with glucose (5 mg/ml) and incubated for another 2 days to the morula/blastocyst stage. No nest is depicted, but the postures are those of the incubating female and the male about to leave in search of food. The zona-free oocytes from each species were incubated with each protein in at least two separate trials. The embryos were incubated until 11 h post-fertilisation. Then, after washing, the embryos were incubated with the second antibody for l h at room temperature. Slides were incubated in the dark for 1 h with an antibody to -tubulin directly labelled with fluorescein. Approximately 1 h before manipulation, the cells were treated as described above and then incubated at room temperature. In a second set of controls, sections were incubated with preimmune serum in place of the primary antibodies. Cells were incubated for another 24 h before electrophysiological experiments. As control, some sections were incubated for the same time in 70% ethanol. Samples were incubated at room temperature for 5 min and placed in the magnetic stand for resin separation. The coelomic oocytes thus obtained were incubated with and without catecholamines in the same conditions as the ovarian oocytes. Pieces of ovary from females captured during the quiescent and breeding periods were incubated in the medium in the presence of the drugs under study. Merozoites were incubated with various sugars for 5 min before invasion was assayed in the continued presence of the sugar. Values are expressed relative to a control mixture which was incubated with no trypsin or inhibitor. Annular alpha-synuclein protofibrils are produced when spherical protofibrils are incubated in solution or bound to brain-derived membranes. Tachyzoites (4i10%) were incubated for 30 min in cell culture medium with decreasing concentrations of serum. Results showed that cells that were infected with vaccinia virus moved more rapidly than cells incubated without virus ('mockinfected' cells). Therefore, a caseininfusion zymogram assay was also used, in which proteinases were first separated and then incubated with casein substrate in solution. At each temperature five sets of approximately 150 eggs were incubated on moist filter paper in petri dishes. The bacterial cultures are incubated and examined within twenty-four hours. Reaction mixtures were incubated between 3 and 16 h at the appropriate temperature. The cells were then incubated for a further 72 hours. Sections were then incubated with primary antibodies in blocking solution overnight at room temperature. A significant immunorecognition was retained when p64 was incubated with acid. Scanning electron microscopy of intact trophozoites incubated with accessory factor showed typical crescent-shaped organisms with clear polar structures. Washed worms were incubated in three changes of 0.9% saline (2 h per change) at room temperature. The percentage reduction observed in faecal pats incubated at low humidity was high throughout the experiment (83- 94%). Cultures of strains on agar plates were incubated from 14 to 20 days. Samples were incubated (' pulsed ') for 60 min (1300-1400 h) to obtain sufficient counts. There was no apparent growth of fungus in faecal pats incubated at a high humidity and less than 20% of larvae were recovered. In several experiments live ticks were collected from a fungus-infested field or from their vertebrate hosts and incubated under optimal laboratory conditions. As a control, sections were incubated with secondary antibody alone. I am burying a dream-bone, leaving it for later, incubating an innuendo, lying it real, reeling it in right. Astroglial cells were subcultured after 10 days and then incubated for 2 weeks. A second control mixture was incubated with a pre-mixed solution of trypsin plus inhibitor. One control mixture was incubated without trypsin or inhibitor. The diaphragm was removed, minced and incubated by the standard technique to recover schistosomula present in the tissues. The diaphragms were removed, minced and incubated, and the hepatic portal system was perfused to recover any worms present in the two locations. If incubated with many of the substances it excretes, the worm will take them up and transform them. After a saturation step, sections were incubated overnight with the primary antibody. As negative control (no histolytic activity), fibroblasts were incubated under identical conditions but without ciliates. The tissues in panels a and c were incubated with anti-a, those in panels b and d were incubated with anti-fl. There was also an absence of staining in the control sections incubated without the primary antibody. Sections were incubated in secondary antibody for 1 h at room temperature. Spermatozoa from the washed and unwashed groups were incubated in solubilised zonae (5 zonae/jil) for 30-40 min and then used for subzonal microinjection. The gas phase in the syringe was replaced with acetylene (10% v/v) and the syringe was incubated for 25 mins. Four preparations were used per experiment, with each preparation incubated in a different concentration of drug. After repeated washing in blocking solution, the blot was incubated in secondary antibody for 3- 4 h at room temperature. To test this, the spot was scraped from the plate, and again incubated with trypsin and separated in two dimensions. The pollen is then incubated in solution and the amino acid-rich solution is ingested. The intact seeds were incubated in tetrazolium chloride, and the stained embryos were considered viable. Pieces of ovary were incubated for 1 h in the incubation medium with and without catecholamines. Controls were incubated for the same period without the extract. Proteins were biotinylated, and incubated with zona-free oocytes. Eggs were incubated for 30-60 min, monitored frequently and activated eggs discarded. Percentages are cumulative for seeds incubated in light. The samples were incubated for different times (5, 10, 20 min) at room temperature. The eggs were incubated in the media containing different drugs for the entire period of culture. The samples were incubated for 30, 60 or 120 min (experiment 1a, b) or for 4 h (experiment 1c, d). The schistosomula suspensions were incubated for between 20 and 24 h and washed the following day before the schistosomula were observed under fluorescent microscopy. The radiolabelled target cells are then incubated with the test effector-cell population for a short period (4-6 hours). Plates were incubated for one hour at room temperature and then washed three times. After two additional 5-minute washes, the slides were incubated for 30 minutes in the solution containing the avidin-biotin-peroxidase complex. An additional 100 l/well complete medium were added, and the cells were incubated until growth was noted, 10 days later. Nitrocellulose membranes were then incubated with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody for 1 hour and washed as above. After 15 minutes of incubation, the agonist was added and the cells were incubated with both drugs for an additional 10 minutes. Membranes were then incubated for 60 minutes in the presence of a goat-anti-mouse secondary antibody linked to horseradish-peroxidase at room temperature. Here, the samples were incubated on 1.5% hard agar surfaces whereas the previous studies used glass or nitrocellulose surfaces. Washed cells were incubated in the following solutions under various temperature/time regimens. The mixture was incubated at room temperature for 20 min. The samples were incubated, washed and dried as described above. Only weak aggregation of cells was observed when cultures were incubated at room temperature for a similar time span. Faeces were incubated for 2, 3, 4 and 5 weeks and then examined with respect to the development and survival of eggs and larvae. The pats were incubated at low or high humidity. In the present trial, faecal cultures incubated for 14 days showed more consistent reduction percentages, compared to cultures incubated only for 7 days. These examples are from corpora and from sources on the web. Any opinions in the examples do not represent the opinion of the Cambridge Dictionary editors or of Cambridge University Press or its licensors. |
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